Specificity: Recognize α-tubulin from Human, Mouse, Tetrahymena, dictyostelium, Saccharomyces.
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IF
Description
Western blot at dilution of 1:1000 and Immunofluorescence staining at 1:100 dilutions. It is very useful as a loading control for quantitive western blotting. The antibody reconizes a 50kDa band in a Western Blot analysis., WB ; IF
Dilution
WB
1:1000
IF
1:100
Reference
Baek, KH et al. "Thrombospondin-1 mediates oncogenic Ras-induced senescence in premalignant lung tumors." J. Clin. Invest. 123:4375-89 (2013). PubMed: 24018559.
Bernasconi, R et al. "Autoadaptive ER-associated degradation defines a preemptive unfolded protein response pathway." Mol. Cell 52:783-93 (2013). PubMed: 24239290.
Byongsun, L et al. "Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2:303-313 (2017). DOI: 10.1016/j.bbamcr.2016.11.013. Application: Loading Control Antibody.
Park, IH et al. "Inhibition of hepatitis B virus replication by ligand-mediated activation of RNase L." Antiviral Res. 104:118-27 (2014). PubMed: 24509240.
Reggiori, F et al. "Coronaviruses Hijack the LC3-I-positive EDEMosomes, ER-derived vesicles exporting short-lived ERAD regulators, for replication." Cell Host Microbe 7:500-8 (2010). PubMed: 20542253.
Reviews of Anti-Alpha-Tubulin Monoclonal Loading Control Antibody