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Zebrafish Testicular Feeder Cells (ZtA6-10)

SKU : BHC10901983

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T2609
Description	ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. Clones were analyzed for the expression of the Sertoli cell marker (Sox9a), the germ cell marker (Vas), and the Wilm? tumor suppressor marker (WT1). 12 clones were derived and have distinctive properties and are available at abm.
Cell Type	Stable Cell Lines
Biosafety Level	2
Depositor	National Institute of Genetics (ROIS/NIG)
Disclaimer	1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each cell line. If you have any questions regarding this, please contact us at [email protected]. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s). 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period".
Donor History	Zebrafish (D. rerio)
Growth Properties	Adherent, epithelial and fibroblast-like
Growth Conditions	Use of PriCoat?T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow XIII Medium (TM013) + 2mM L-Glutamine (G275) + 100 ug/mL Kanamycin sulfate + 800 uM CaCl2 + 200 ug/mL L-arginine +20 ug/mL aspartic acid + 15ug/ul L-histidine- HCl + 72.5 ug/mL L-lysine-HCl + 20 ug/mL L-proline + 0.5% w/v Bovine serum albumin + 10mM HEPES + 10 IU/mL human chorionic gonadotropin + 10 IU/mL pregnant mare's serum gonadotropin + 3% FBS. Sterilize by filtration (0.2 um filter), store at store at 4? and should be used within 2 weeks. , 38.0?, no CO?. Use only Accutase and Gelatin coated dishes.
Quantity	1x10⁶ cells/ml
Tissue	Testes
Morphology	Epithelial-like\|Fibroblast-like
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Fish
Species	Zebrafish (D. rerio)
Propagation	Grow cells in T25 gelatin-coated flasks (TM063) with the following conditions. The base medium for this cell line is L-15 medium (ThermoFisher Scientific). To make the completed growth medium, add the following components to the base medium at the following final concentrations: 5 IU/ml human chorionic gonadotropin (Sigma), 2 IU/ml pregnant mares serum gonadotropin (Sigma), 0.2 mg/ml L-arginine (Gibco BRL), 0.02 mg/ml L-aspartic acid (Gibco BRL), 0.015 mg/ml L-histidine (Gibco BRL), 0.0725 mg/ml L-lysine HCl (Gibco BRL), 0.02 mg/ml L-proline (Gibco BRL), 0.5% bovine serum albumin (5% w/v stock solution, BSA fraction V, Sigma), 1% Hepes (Sigma), fetal bovine serum (TM999) to a final concentration of 3%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Atmosphere: air: 100%, Temperature: 28.0°C. To prepare as feeder cells: Treat with 10 µg/ml mitomycin C in L-15 for 5 hours before plating for use as feeder cells for further experimentation.
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Source Organ	Reproductive
Quality Control	1) Phagocytic activity was analyzed via the ability to internalize polystyrene beads; 2) RT-PCR was used to assess the presence or absence of WT1, Vas, and Sox9a markers; 3) Functionality test was performed to determine the ability to support male germ cells when co-cultured as feeder cells.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Subculture Protocol	Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37?, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10901983	T2609	1x10^6 cells / 1.0 ml	-		Add to Quote

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