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TRPC7-HEK293 Cell Line

SKU : BHC18200229

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BPS Bioscience

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Mfr.No.	90030
Description	Stable, recombinant HEK293 cell line expressing human TRPC7 (transient receptor potential cation channel, subfamily C, member 7, accession number NM_020389). Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Application	Monitor TRPC7 calcium channel activity Screen for activators or inhibitors of TRPC7 calcium channel
Biological Activity	N-terminal FLAG tagged human TRPC7 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC7 was characterized by calcium assay. In TRPC7-HEK293 cells, addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC7, resulting Ca²⁺ entry in the cells.
Host Cell	HEK293
Uniprot	Q70T24
Species	Human
Synonyms	TRPC7, transient receptor potential cation channel, subfamily C, member 7
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Moran MM et al. (2011). Nat Rev Drug Discov. 10(8):601-620 2. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol. 87: 100-107 3. Lievremont J., et al. (2004). Am J Physiol Cell Physiol 287: C1709-C1716.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200229	90030	2 vials	$10340		Add to Cart

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