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TRPC3-HEK293 Cell Line

SKU : BHC18200230

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BPS Bioscience

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Mfr.No.	90130
Description	Stable, recombinant HEK293 cell line expressing human TRPC3 (transient receptor potential cation channel, subfamily C, member 3, accession number NM_003305). Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Background	TRPC3 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRP channels share a similar architecture of six-transmembrane domains and function as tetramers. Most channels are permeable to calcium and monovalent cations (Vassort et al., 2009). Specifically, TRPC3 channel belongs to a canonical TRP (TRPC) subfamily that is involved in calcium entry. TRPC3 is activated through phospholipase C-linked receptors. When expressed in HEK293 cells, TRPC3 behaves as a receptor activated channel with constitutive activity that cannot be further increased by store depletion (Trebak et al., 2002).
Application	Monitor TRPC3 calcium channel activity Screen for activators or inhibitors of TRPC3 calcium channel
Biological Activity	N-terminal FLAG tagged human TRPC3 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC3 was characterized by calcium assay. TRPC3 produces a constitutive Ca²⁺ entry in HEK293 cells. Addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC3.
Host Cell	HEK293
Uniprot	Q13507
Species	Human
Synonyms	TRPC3, Transient Receptor Potential Cation Channel, Subfamily C, Member 3
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Albert A.P., Saleh S.N., and Large W.A. (2009). Current Medicinal Chemistry 16: 1158-1165. 2. Lievremont J., et al. (2004). Am J Physiol Cell Physiol 287: C1709-C1716. 3. Trebak M., et al. (2002). J. Biol. Chem. 277: 21617-21623. 4. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol. 87: 100-107.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200230	90130	2 vials	$10340		Add to Cart

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