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Transferrin Receptor Protein-1 (TFRC) HEK293 Recombinant Cell Line

SKU : BHC18200098

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BPS Bioscience

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Mfr.No.	78308
Description	Recombinant clonal HEK293 stable cell line constitutively expressing full length human Transferrin Receptor Protein-1 (TFRC and TFR1, also known as human CD71 protein, GenBank accession #NM_001128148). The surface expression of TFRC was confirmed by flow cytometry. Mycoplasma testing: The cell line has been screened using the MycoAlert Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~2 x 10⁶ cells in 1 ml of 10% DMSO
Background	Transferrin Receptor Protein-1 (TFRC) is a cell surface receptor that binds ferric-iron-loaded transferrin in the bloodstream at high affinity to facilitate iron uptake into cells. TFRC is therefore an integral component of the body's iron supply chain and general homeostasis. Intracellular iron levels, hypoxia and CRE signaling regulate TFRC transcription. In addition, TFRC is upregulated in correlation with tumor-progressive stages of multiple forms of cancer. At the cellular level iron surplus results in oxygen radical formation and cellular dysfunction, while iron deficiency can cause rapid cell death. Deregulated iron levels are therefore correlated with multiple disease states including Parkinson's Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease, Multiple Sclerosis (MS), and tumorigenesis. Equally important are potential therapeutic uses of TFRC. As TFRC is upregulated on the surface of tumor cells, it has been targeted to inhibit adult T-cell leukemia/lymphoma. Additionally, TFRC has found important utility in treatments of neurological diseases. Drug discovery in the central nervous system (CNS) space has historically been challenged by limited access of therapeutics across the blood brain barrier (BBB). As TFRC is highly expressed on the surface of brain capillary endothelial cells within the BBB, drug delivery strategies are being developed to leverage transcytosis of TFRC-bound entities from the bloodstream to the CNS (i.e. receptor mediated transport, or RMT). Current approaches include PEGylated liposomes coated with the TFRC ligand transferrin, as well as anti-TFRC antibodies linked to nanoparticles (immunoliposomes) carrying a therapeutic payload.
Application	1. Useful for screening or performing binding assays of antibodies designed against human Transferrin Receptor-1 in a cellular context. 2. Study of endocytosis kinetics on transferrins and their derivatives in cellular uptake assays for the development of next generation culture media formulations. 3. Preliminary assessment of transferrin receptor-targeted nanoparticle complexes (immunoliposomes) designed to deliver therapeutic payloads across the blood brain barrier (BBB) via transcytosis.
Formulation	Thaw Medium 1 (Biohippo #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS, 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).Growth Medium 1C (Biohippo #79532): Thaw Medium 1 (Biohippo, #60187) plus 50ug/ml of Hygromycin B to ensure stability of expression.Assay Medium: Thaw Medium 1 (Biohippo, #60187)
Host Cell	HEK293
Species	Human
Synonyms	T9, TR, TFR, p90, CD71, TFR1, TRFR, IMD46
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200098	78308	2 vials	$10340		Add to Cart

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