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TMEM16B (ANO2) - HEK293 Recombinant Cell line

SKU : BHC18200234

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BPS Bioscience

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Mfr.No.	90332
Description	Recombinant HEK293 cell line expressing human TMEM16B (transmembrane protein16B, also called as anoctamin 2, calcium-activated chloride channel (ANO2), accessionnumber NM_001278596). Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM MycoplasmaDetection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Background	Calcium-activated chloride channels (CaCCs) are involved in a variety of physiological functions including smooth muscle contraction and olfaction. TMEM16B (ANO2) has been identified as a CaCC that is activated by intracellular Ca²⁺ and Ca²⁺-mobilizing stimuli. It has eight putative transmembrane segments and is permeable to monovalent anions.
Application	Drug compound screening for activators or inhibitors of TMEM16B Functional assays for TMEM16B Efficient antigen for mouse immunization
Biological Activity	Human TMEM16B channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16B was characterized by an assay based on a halidesensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16B-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca²⁺, TMEM16B produced I- influx in HEK293, triggering a rapid decrease of fluorescence by the transfected YFP mutant. The ionomycin-induced I- influx through TMEM16B was blocked by niflumic acid, a CaCC channel blocker.
Host Cell	HEK293
Uniprot	19475416
Species	Human
Synonyms	TMEM16B, transmembrane protein 16B, anoctamin 2, calcium-activated chloride channel, ANO2
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. NatureReviews 8:153-171.2. Scudieri P. et al. (2012) The anoctamin family: TMEM16A and TMEM16B as calciumactivatedchloride channels. Exp. Physiol. 97(2):177-183.3. StA hr H. et al. (2009) TMEM16B, a novel protein with calcium-dependent chloridechannel activity, associates with a presynaptic protein complex in photoreceptorterminals. J. Neurosci. 29(21):6809-6818.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200234	90332	2 vials	$10340		Add to Cart

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