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SRE Reporter - HEK293 Cell Line (ERK Pathway)

SKU : BHC18200004

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BPS Bioscience

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Mfr.No.	60406
Description	The SRE Reporter - HEK293 cell line is designed for monitoring the activity of the JAK/STAT signaling pathway. The SRE Reporter - HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Background	The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
Application	Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity. Screen for activators or inhibitors of the MAPK/ERK signaling pathway.
Biological Activity	This cell line is validated for the response to the stimulation of EGF or serum and to the treatment of inhibitors of ERK signaling pathway.
Host Cell	HEK293
Synonyms	SRE cell line, MAPK reporter, ERK reporter
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Product Format	aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Wong, K.K. (2009) Recent developments in anti-cancer agents targeting the Ras/Raf/ MEK/ERK pathway. Recent Pat. Anticancer Drug Discov. 4(1):28-35. 2. Treisman, R. (1992) The serum response element. Trends Biochem. Sci. 17(10): 423-426

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200004	60406	2 vials	$2755.50		Add to Cart

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