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SRE eGPF Reporter - HEK293 Cell Line (ERK Pathway)

SKU : BHC18200105

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Mfr.No.	78327
Description	Recombinant HEK-293 cells expressing enhanced green fluorescent protein (eGFP) under the control of SRE responsive elements. This cell line is validated for its response to EGF or serum stimulation and to treatment with inhibitors of ERK signaling pathway. Purity: SRE cell line, MAPK reporter, ERK reporter Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as 2 vials: Each vial contains 2 x 10⁶ cells in 1 ml of 90% FBS, 10% DMSO
Background	The MAPK/ERK signaling pathway is a key regulator of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylates and activates ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The Ternary Complex Factors (TCFs), including Elk1, are among the best-characterized transcription factor substrates of ERK1/2. When phosphorylated by ERK1/2, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
Application	Screen for compound effect on the MAPK/ERK signaling pathway. Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
Formulation	Thaw Medium 1 (Biohippo #60187): MEM medium (Thermo Fisher, #11095098) supplemented with 10% FBS, 1% non-essential amino acids (Corning, #25-025-CI), 1 mM Na pyruvate (Corning, #25-000-CI), 1% Penicillin/Streptomycin (Thermo Fisher, #15140163)Growth Medium 1J (Biohippo #79552): Thaw Medium 1 (Biohippo #60187), 500 ug/ml of Geneticin/G418Assay Medium 1: Thaw Medium 1 (Biohippo #60187)Assay Medium 1B (Biohippo #79617): MEM medium (Thermo Fisher, #11095098) supplemented with 0.5% FBS, 1% non-essential amino acids (Corning, #25-025-CI), 1 mM Na pyruvate (Corning, #25-000-CI), 1% Penicillin/Streptomycin (Thermo Fisher, #15140163)
Host Cell	HEK293
Species	Human
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles. Do not use a -80°C freezer for long term storage.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200105	78327	2 vials	$2502.50		Add to Cart

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