Rat Primary Ovarian Smooth Muscle Cells from Cell Biologics are isolated from the Ovarian tissues of 6-8 weeks old laboratory SpragueDawley rat and grown in gelatin pre-coated tissues culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Rat Primary Ovarian Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of ?-smooth muscle actin (A2547, Sigma) and are negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin
Rat
Cell Type
Smooth Muscle Cells
Application
Rat Primary Ovarian Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer
Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen
1×106cells
General Information
Rat Primary Ovarian Smooth Muscle Cells are characterized by immunofluorescent method with antibody to ?-smooth muscle actin. Rat Primary Ovarian Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics). Repeated freezing and thawing of cells is not recommended.
Media
Complete Smooth Muscle Cell Medium w/ Kit 500 ml
Tissue
Ovarian
Notes
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species
Rat
Applications
Urogenital Research
Product Use
Rat Primary Ovarian Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.