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Rat Primary Dermal Epithelial Cells

SKU : BHC16401145

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Cell Biologics, Inc.

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Mfr.No.	RA-6066
Description	Rat Primary Dermal Epithelial Cells from Cell Biologics are isolated from the skin tissues of 6-8 weeks old laboratory SpragueDawley rat and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. Rat Primary Dermal Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (sc-8426, Santa Cruz) or ZO-1 (MABT11, clone R40.76, Millipore) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Rat
Cell Type	Epithelial Cells
Application	Standard biochemical procedures performed with epithelial cell cultures include the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	0.5×10⁶cells
General Information	Rat Primary Dermal Epithelial Cells are characterized by immunofluorescent staining with antibody of E-cadherin (Anti-E-Cadherin Antibody, sc-8426, Santa Cruz) or ZO-1 (Anti-ZO-1 Antibody, MABT11, clone R40.76, Millipore). Rat Primary Dermal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Media	Complete Epithelial Cell Medium w/ Kit 500 ml
Tissue	Dermal
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Rat

Applications	Dermatologic Research

Product Use	Rat Primary Dermal Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401145	RA-6066	0.5x106 Cells	$510		Add to Cart

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