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Home / Cells / Primary Cells

Rat Primary Coronary Artery Fibroblasts

SKU : BHC16401176

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Cell Biologics, Inc.

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Mfr.No.	RN-6049CO
Description	Rat Primary Coronary Artery Fibroblasts from Cell Biologics are isolated from coronary artery tissue of 1-day-old neonatal Sprague-Dawley rats. Rat Primary Coronary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 2-5 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶ cells per ml and is delivered frozen.
Cell Origin	Rat
Cell Type	Fibroblasts
Application	Rat Primary Coronary Artery Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although Rat Primary Coronary Artery Fibroblasts are isolated from laboratory rats testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	Rat Primary Coronary Artery Fibroblasts from Cell Biologics are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Rat Primary Coronary Artery Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Coronary

Product Use	Rat Primary Coronary Artery Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request.Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401176	RN-6049CO	Frozen Vial	$310		Add to Cart

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