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Rat Primary Cholangiocytes

SKU : BHC16401138

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Cell Biologics, Inc.

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Mfr.No.	RA-6044C
Description	Rat Primary Cholangiocytes from Cell Biologics are isolated from the bile duct tissue of 6-8 week old laboratory SpragueDawley rat. Rat Primary Cholangiocytes are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x10⁶ cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin	Rat
Application	Standard biochemical procedures performed with epithelial cell cultures include the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	0.5×10⁶cells
General Information	Rat Primary Cholangiocytes are characterized by immunofluorescent staining with antibody of E-cadherin (Anti-E-Cadherin Antibody, sc-8426, Santa Cruz) or ZO-1 (Anti-ZO-1 Antibody, MABT11, clone R40.76, Millipore). Rat Primary Cholangiocytes are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Product Use	Rat Primary Cholangiocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401138	RA-6044C	Frozen Vial	$510		Add to Cart

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