Rat Brain Microvascular Endothelial Cells (RBMEC)

Product Description

Brain microvascular endothelial cells (BMEC), the major component of the blood-brain barrier, limit the passage of substances, both soluble and cellular, from the blood into the brain. BMEC utilize unique features to distinguish themselves from peripheral endothelial cells, such as 1) intercellular tight junctions that display high electrical resistance and slow paracellular transport, 2) the absence of fenestrae and a reduced level of pinocytic activity, and 3) the expression of specialized pumps that can transport compounds out of the brain via the blood-brain barrier (1-3). Similar to peripheral endothelial cells, BMEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. Cultured rat BMEC have been widely used for studying the molecular and cellular properties of blood-brain barrier because of their unique functions. Understanding the molecular mechanisms of blood-brain barrier regulation may help to optimize drug delivery to the CNS and elucidate new therapies for CNS diseases.

iXCells Biotechnologies provides high quality Rat Brain Microvascular Endothelial Cells (RBMEC), which are isolated from adult rat brain and cryopreserved at P1, with >0.5 million cells in each vial. RBMEC express vWF/Factor VIII and CD31 (PECAM). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies. 

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References

(1) Crone C, Oleson SP. (1992) "Electrical resistance of brain microvessel endothelium." Brain Res. 241: 49-55. 
(2) Reese TS, Karnovsky MJ. (1967) "Fine structural localization of blood-brain barrier to exogenous peroxidase." J Cell Biol. 34: 9-14. 
(3) Wolburg H, Neuhaus J, Kniesel U, Kraub B, Schmid EM, Ocalan M, Farrell C, Risau W. (1994) "Modulation of tight junction structure in blood-brain barrier endothelial cells." J Cell Sci. 107: 1347-1357.

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