The RARbeta Reporter (Luc)-HEK293 cell line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARbeta (accession # P10826-2).
Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Application
The RARbeta Reporter (Luc)-HEK293 cell line is designed for monitoring the activity of RARbeta. Monitor RARbeta-regulated pathway activity Screen agonists or antagonists of RARbeta.
Biological Activity
This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARbeta is confirmed by Western blotting.
Host Cell
HEK293
Uniprot
P10826-2
Synonyms
RAR beta, Retinoic acid receptor beta, RARB
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.
Product Format
aqueous solution containing DMSO
Storage Stability
Store in liquid nitrogen immediately upon receipt.
Reference
1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450. 2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.