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RARalpha Reporter Cellular Assay Pack

SKU : BHC18200178

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BPS Bioscience

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Mfr.No.	79322
Description	The RARalpha Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor alpha (RARalpha). The pack contains the RARalpha Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARalpha (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARalpha is confirmed by Western blotting.Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. Host Species: human
Background	Retinoic acid receptor (RAR) belongs to a family of nuclear receptors and has three subtypes, RARalpha, RARbeta, and RARgamma. RAR heterodimerizes with RXR (retinoid X receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes.
Application	Monitor RARalpha-regulated pathway activity Screen agonists or antagonists of RARalpha.
Host Cell	HEK293
Synonyms	RAR Alpha, NCOR2, SMRT, RARA, Retinoic Acid Receptor Alpha
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Immediately upon receipt, store vials in liquid nitrogen.

Reference	1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450.2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200178	79322	2 vials	$2629		Add to Cart

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