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Home / Cells / Primary Cells

Rabbit Primary Kidney Glomerular Endothelial Cells

SKU : BHC16401543

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Cell Biologics, Inc.

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Mfr.No.	N-6014G
Description	Rabbit Primary Kidney Glomerular Endothelial Cells fromCell Biologics are isolated from kidney glomerular tissue of New Zealand White Rabbit. Rabbit Primary Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and are delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic pre-coated with secondary antibody.
Cell Origin	Rabbit
Cell Type	Endothelial Cells
Application	Rabbit Primary Kidney Glomerular Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Frozen	0.5-1×10⁶cells
General Information	Rabbit Primary Kidney Glomerular Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Rabbit Primary Kidney Glomerular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Kidney

Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401543	N-6014G	Frozen Vial	$610		Add to Cart

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