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PDE7A-HEK293 Cell Line

SKU : BHC18200005

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Mfr.No.	60407
Description	Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells, inducing the expression of the luciferase reporter, whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Background	Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP byhydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclicnucleotides play an important role as second messengers in diverse physiologicalfunctions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B)have been identified. PDE7 is widely expressed in various tissues, with PDE7A foundprimarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7Bare detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads toelevated intracellular level of cAMP.
Application	Useful to monitor cAMP and PDE7A signaling pathway activity and to screen activators or inhibitors of PDE7A.
Host Cell	HEK293
Uniprot	Q13946
Species	Human
Synonyms	PDE7A, phosphodiesterase 7A
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Product Format	aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Malik, R. et al. (2008) Appl. Microbiol. Biotechnol. 77 (5): 1167-1173. 2. Fan Chung, K. (2006). Eur. J. Pharmacol. 533(1-3):110-117.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200005	60407	2 vials	$10197		Add to Cart

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