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PDE10A-HEK293 Cell line

SKU : BHC18200008

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Mfr.No.	60410
Description	Recombinant HEK293 cell line expressing human PDE10A2 (phosphodiesterase 10A, accession number NM_006661). N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Background	Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP byhydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclicnucleotides play an important role as second messengers in diverse physiologicalfunctions. PDE10A plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE10A hydrolyzes both cAMP and cGMP to nucleoside 5 -monophosphate, but exhibits higher affinity for cAMP, and it utilizes cAMP more efficiently as a substrate (Fujishige K. et al., 1999).
Application	Useful to monitor cAMP and PDE10A signaling pathway activity and to screen activators or inhibitors of PDE10A.
Host Cell	HEK293
Uniprot	Q9Y233
Species	Human
Synonyms	PDE10A, phosphodiesterase 10A
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Product Format	aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200008	60410	2 vials	$10197		Add to Cart

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