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NFAT Reporter (Luc) - Jurkat Cell Line

SKU : BHC18200046

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BPS Bioscience

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Mfr.No.	60621
Description	The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of theNFAT response element stably integrated into Jurkat cells. This cell line has been validated forresponse to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful asa control cell line for other NFAT reporter cell lines expressing various immune checkpointreceptors. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit(Sigma-Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~2x106 cells in 1 ml of 10% DMSO.
Background	The nuclear factor of activator T cells (NFAT) family of transcription factors plays an importantrole in immune response. T cell activation through the T cell synapse results in calcium influx.Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin,which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the aminotermini of NFAT proteins. This results in a conformational change that exposes a nuclearlocalization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperatewith other proteins to bind to DNA.
Application	Monitor intracellular calcium levels. Screen for activators or inhibitors of PKC/ Ca2+ pathway. Screen for agonists or antagonists of T cell receptors. Control for immune checkpoint NFAT reporter cell lines.
Host Cell	Jurkat
Species	Human
Synonyms	Nuclear factor of activated T cells; T cell
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Immediately upon receipt, store vials in liquid nitrogen.

Reference	1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7. 2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200046	60621	2 vials	$2524.50		Add to Cart

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