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Home / Cells / Cell Lines

NF-kappaB reporter (Luc) - NIH/3T3 Cell line

SKU : BHC18200191

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BPS Bioscience

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Mfr.No.	79469
Description	The NF-kappaB reporter (Luc)-NIH/3T3 cell line is designed for monitoring nuclear factor Kappa B (NF-kappaB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-kappaB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-kappaB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species. Host Species: mouse Supplied as Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO
Application	Monitor NF-kappaB signaling pathway activity. Screen for activators or inhibitors of NF-kappaB signaling pathway.
Host Cell	NIH/3T3
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Immediately upon receipt, store in liquid nitrogen.

Reference	1. Pessara U, Koch N (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-kappaB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.2. Baeuerle PA (1998) Pro-inflammatory signaling: last pieces in the NF-kappaB puzzle Curr Biol. 8(1):R19-R22.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200191	79469	2 vials	$2090		Add to Cart

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