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NF-kappaB reporter (Luc) - HEK293 Cell line

SKU : BHC18200053

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Mfr.No.	60650
Description	The NF-kappaB reporter (Luc) HEK293 cell line is designed to monitor nuclear factor Kappa B (NF-kappaB) activity. It contains a firefly luciferase gene driven by four copies of the NF-kappaB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-kappaB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been functionally validated in response to human TNF-alpha, IL-1beta, and IL-17. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Background	Nuclear factor-kappaB (NF-kappaB)/Rel proteins include NF-kappaB2 p52/p100, NF-kappaB1 p50/p105, c-Rel, RelA/p65, and RelB. These proteins function as dimeric transcription factors that control genes regulating a broad range of biological processes including innate and adaptive immunity, inflammation, stress responses, B cell development, and lymphoid organogenesis. In the classical (or canonical) pathway, NF-kappaB/Rel proteins are bound and inhibited by IkappaB proteins. Proinflammatory cytokines, LPS, growth factors, and antigen receptors activate an IKK complex (IKKbeta, IKKalpha, and NEMO), which phosphorylates IkappaB proteins. Phosphorylation of IkappaB leads to its ubiquitination and proteasomal degradation, freeing NF-kappaB/Rel complexes. Active NF-kappaB/Rel complexes are further activated by phosphorylation and translocate to the nucleus where they induce target gene expression. In the alternative (or noncanonical) NF-kappaB pathway, NF-kappaB2 p100/ RelB complexes are inactive in the cytoplasm. Signaling through a subset of receptors, including LTbetaR, CD40, and BR3, activates the kinase NIK, which in turn activates IKKalpha complexes that phosphorylate C-terminal residues in NF-kappaB2 p100. Phosphorylation of NF-kappaB2 p100 leads to its ubiquitination and proteasomal processing to NF-kappaB2 p52, creating transcriptionally competent NF-kappaB p52/RelB complexes that translocate to the nucleus and induce target gene expression.
Application	The NF-kappaB reporter cell line is designed for screening inhibitors of NF-kappaB and for monitoring NF-kappaB signaling pathway activity.
Biological Activity	This cell line is validated for the response to TNFalpha and to treatment with NF-kappaB inhibitor, evodiamine.
Host Cell	HEK293
Species	Human
Synonyms	NF-kappaB cell line, NFkappaB cell line, luciferase reporter, NF-kappaB cell line, NFkB cell line
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Product Format	aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Pessara U, Koch N (1990). Mol Cell Biol. 10(8):4146-4154. 2. Baeuerle PA (1998). Curr Biol. 8(1):R19-R22. 3. Takada Y, Kobayashi Y, Aggarwal BB (2005). J Biol Chem. 280(17):17203-17212.Application Reference(s): Synthesis of novel C5-curcuminoid-fatty acid conjugates and mechanistic investigation of their anticancer activity (2015)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200053	60650	2 vials	$1501.50		Add to Cart

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