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Mouse Tumor-Associated Endothelial Cells (Mouse Breast Cancer Origin, 4T1)

SKU : BHC16400281

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Cell Biologics, Inc.

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Mfr.No.	MC-6020m
Description	Mouse Tumor-Associated Endothelial Cells (Mouse Breast Cancer Origin, MTMMECM) from Cell Biologics were isolated from pathogen-free laboratory nude mice. MTMMECM were isolated from human xenograft of pathogen-free laboratory nude mice. Mouse breast adenocarcinoma epithelial cells (4T1) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTMMECM were cultured in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials. The method we use to isolate primary tumor-associated endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin	Mouse
Cell Type	Endothelial Cells
Application	MTMMECM can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Disclaimer	Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen	0.5×10⁶cells
General Information	MTMMECM are tested for expression of markers using antibody, Ve-Cadherin (CD144, VE-Cadherin Antibody, C-19, sc6458 Santa Cruz); or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. MTMMECM are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Breast

Product Use	MTMMECM from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400281	MC-6020m	Frozen Vial	$590		Add to Cart

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