Mouse Pulmonary Endothelial Cells (MPEC)

Product Description

Mouse pulmonary endothelial cells are widely used in vascular biology and lung cell biology studies such as pulmonary inflammation (Biotech), angiogenesis, vessel permeability, leukocyte/EC interaction, nitric oxide production, and mechanotransduction ⁽¹⁾.  Endothelial dysfunction is the common molecular basis of multiple human diseases, such as atherosclerosis, diabetes, hypertension, and acute lung injury. Primary culture of ECs is an important tool to dissect the role of endothelial genes in endothelial dysfunction-associated disorders ⁽²⁾. Mouse pulmonary endothelial cells has been successfully used in phenotypic, and genetic studies characterizing endothelial genes in human diseases ⁽³⁾.

iXCells Biotechnologies provides high quality mouse pulmonary endothelial cells (MPEC), which are isolated from peripheral tissues of pulmonary lobes of C57BL/6 or CD1 mouse lung. MPECs are cryopreserved at passage 2 and delivered frozen. Each vial contains >0.5million cells. MPEC are characterized by immunofluorescence with antibody specific to Tie-2. MPECs are negative for mycoplasma, bacteria, yeast, and fungi. MPECs are guaranteed to further expand no more than 2 additional passages in Endothelial Cell Growth Medium (Cat# MD-0010-500ML) under the condition suggested by iXCells Biotechnologies.

Figure 1.  Mouse pulmonary endothelial cells (MPEC).  (A) Phase contrast image of MPEC. (B) Immunofluorescence staining with antibody against Tie-2.

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References

(1) Peramaiyan Rajendran, Thamaraiselvan Rengarajan, Jayakumar Thangavel, Yutaka Nishigaki, Dhanapal Sakthisekaran, Gautam Sethi, Ikuo Nishigaki. (2013) “The vascular endothelium and human diseases.” Int J Biol Sci. 9(10): 1057-69.

(2) Hadi AR Hadi, Cornelia S Carr, and Jassim Al Suwaidi. (2005) “Endothelial Dysfunction: Cardiovascular Risk Factors, Therapy, and Outcome”.  Vasc Health Risk Manag. 1(3): 183-198.

(3) Monica J. Justice, Paraminder Dhillon. (2016) “Using the mouse to model human disease: increasing validity and reproducibility”.  Disease Models & Mechanisms. Editorial.

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