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Home / Cells / Primary Cells

Mouse Colon Tumor-Associated Smooth Muscle Cells (Mouse Colon Cancer Origin, HCT116)

SKU : BHC16400299

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Cell Biologics, Inc.

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Mfr.No.	MC-6001
Description	Mouse Colon Tumor-Associated Smooth Muscle Cells were isolated from pathogen-free laboratory nude mice. Cells were isolated from xenograft of pathogen-free laboratory nude mice. Mouse Colon adenocarcinoma epithelial cells (HCT116) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. Cells were cultured in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 7-14 days. Cells were detached from flasks and immediately cryo-preserved in vials. Cells will be delivered in a frozen vial and each vial contains 1x10⁶ cells/ml.
Cell Origin	Mouse
Cell Type	Smooth Muscle Cells
Application	Mouse Colon Tumor-Associated Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen	0.5×10⁶cells
General Information	Cells are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2-4 passages at a split ratio of 1:2) under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Colon

Product Use	Mouse Colon Tumor-Associated Smooth Muscle Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures, or not for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400299	MC-6001	Frozen Vial	$580		Add to Cart

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