IPC-366 Cells are derived from a triple negative ( ER-, PR-, HER-2-) canine primary inflammatory mammary cancer (IMC) sample that was obtained after the euthanasia of the clinically diagnosed female dog. The morphology of these cells when adhered is epithelial-like and they are positive for general epithelial markers (pancytokeratin, AE1/AE3) as well as the basal marker CK14. Cells also over express E-cadherin and COX-2 and have a high rate of proliferation, but lack p63 and smooth muscle actin. IPC-366 Cells share several characteristics to human derived IMC line, SUM149, including its triple negative nature, and epithelial-like morphology. Due to these similarities, IPC-366 Cells may be useful as a model for study of inflammatory breast cancer.
Cell Type
Tumor Cells
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1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, L-glutamine (G275) to a final concentration of 1%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0
Quality Control
1) Morphology of the IPC-366 Cells (Figure 2). 2) Resin sections viewed through Electron microscope (Figure 3). 3) Tumor growth progression comparison in mice between SUM149 and IPC-366 cells (Figure 3-2 and Table 2). 4) Tumor histology of IPC-366 cells and SUM149 cells in paraffin sections (Figure 4). 5) Immunohistochemistry to detect pancytokeratin, AE1/AE3, CK14, alpha-smooth muscle actin, p63 progesterone receptor, estrogen receptor, HER-2, COX-2 and Ki-67 markers.