Recombinant Jurkat cell line expressing firefly luciferase under the control of an IL-2-responsive promoter, and with constitutive expression of human KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3; GenBank accession #BC143802.1 corresponding to KIR3DL3*00402 allele). HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H) in naA¯ve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains>2 x 106 cells in 1 ml of Cell Freezing Medium (Biohippo, #79796)
Application
Characterize the biological activity of KIR3DL3 interaction with B7-H7 Screen for anti-B7-H7 antibodies in co-culture with B7-H7-expressing cells
Formulation
For best results, it is highly recommended to use these validated and optimized media from Biohippo. Other preparations or formulations of media may result in suboptimal performance.Thaw Medium 2 (Biohippo #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 2P (Biohippo #78354): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.25 ug/ml of Puromycin and 1 mg/ml Geneticin.
Host Cell
Jurkat
Species
Human
Synonyms
Killer cell immunoglobulin-like receptor 3DL3, CD158 antigen-like family member Z, Killer cell inhibitory receptor 1, CD_antigen: CD158z, CD158Z, KIR3DL7, KIRC1
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Reference
1. Bahatt RS, et. al., Cancer Immunol Res. 2021 Feb;9(2):156-169.