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Immortalized Swine Spleen Macrophage Cells - SV40T & t

SKU : BHC10900932

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0141
Description	The Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) were derived from the spinal cord of a trisomy 16 fetal mouse after 12-16 days of gestation. Trisomy 16 in mice is an animal model for trisomy 21, or Down Syndrome, in humans. Since trisomy 16 animals die in utero , the immortalized Trisomy 16 Mouse Neuronal Cells (MTh) offers an immortalized in vitro model. The fetal mouse was bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. RT-PCR found an overexpression of enzyme SOD-1, encoded on mouse chromosome 16 and human chromosome 21, and confirmed the trisomy status of the cell line. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh). Upon comparison to Immortalized Mouse Neuronal Cells (M4b) (Cat. T0674), the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) were found to have a greater basal (Ca2+) and a greater sensitivity to neurotransmitters. No significant difference in choline uptake was observed between the two cell lines. The Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) can be used as an in vitro model to study neuronal biology and physiology related to Down Syndrome.
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Quantity	1x106 cells / 1.0 ml
Tissue	Brain
Morphology	Neuronal
Population Doubling	38 - 48 hours
Species	Mouse (M. musculus)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, Bovine Serum (Sigma) to a final concentration of 10%, 1% L-glutamine (G275) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0
Quality Control	1) RT-PCR for SOD-1 and GAPDH; 2) Immunohistochemistry of neuronal markers; 3) Intracellular Ca2+ measurements;

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