Follicle stimulating hormone (FSH) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately FSH receptor expression is often lost within 1-2 days of in vitro culture of primary cells.The Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor is responsive to FSH stimulation and produces progesterone as a result at levels comparable to primary cells. This cell line is useful in studying FSH-FSH receptor interactions, as well as how FSH receptor regulates the development and maturation of ovarian follicle. Together with the Immortalized Rat Steroidogenic Granulosa Cells expressing LH Receptor (Cat. No T0606), researchers can compare the LH and FSH signaling in homologues cell systems.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Quantity
1x106 cells / 1.0 ml
Tissue
Reproductive
Morphology
Multi-Polar
Population Doubling
35 - 45 hours
Species
Rat (R. norvegicus)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1% Carbon dioxide (CO2): 5%, Temperature: 37.0* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control
1) Phenotypic response to FSH was characterized by shape changes (cells rounding up); 2) FSH receptor expression was measured by radiolabelled 125I-oFSH binding assay- the cells express 27,000 FSH receptors/cell with a Kd of 100-115 pM, similar to the native receptor; 3) Production of progesterone was determined by radioimmunoassay (RIA); 4) Production of cAMP was measured by a protein binding method
Reviews of Immortalized Rat Skeletal Myoblast Cells (L6)