JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Cells / Cell Lines

Immortalized Rat Cochlear Microglia Cells (Mocha)

SKU : BHC10900890

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Applied Biological Materials (abm) Inc.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Description
Review(s)

Mfr.No.	T0740
Description	The Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) were derived from primary conjunctival epithelial cells from Fischer 344 rats. The primary cells were immortalized with pSV(neo) and transfected with SV40 large T antigen. The immortalized cells display epithelial morphology and express the conjunctiva-specific cytokeratin 4, the goblet cell-specific cytokeratin 7 and were negative for the corneal epithelial cell-specific cytokeratin 12. The Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) is useful as models for studying the conjunctival epithelial barrier and also in developing strategies for delivering macromolecular antigens across this barrier to underlying components of the immune system.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	Cytokeratin 4, Cytokeratin 7
Quantity	1x106 cells / 1.0 ml
Tissue	Eye
Morphology	Epithelial-like
Population Doubling	48 - 60 hours
Species	Rat (R. norvegicus)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.PriGrow IV (TM004) + 0.5% BSA + 10 mM HEPES (TM058) + X trace elements B + 10 mug/ml insulin (TM053) + 5.5 mug/ml transferrin + 50 ng/ml sodium selenate + 20 mug/ml ethanolamine + 0.3 mug/ml glucagon + 0.1 muM dexamethasone + 0.1 muM retinoic acid + 0.3 muM 3, 3, 5 triiodo-L thyronine + 80 ng/ml EGF + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?To subculture use TrypLE Express instead of Trypsin-EDTA.
Quality Control	1) Immunofluorescence confocal microscopy demonstrating cytokeratin expression

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Your price	Quantity
BHC10900890	T0740	$490		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.