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Immortalized Preadipocyte Clonal Cell Line SCF3-1; Type 2

SKU : BHC10900854

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0806
Description	Previously immortalized sertoli cells- involved in androgen regulation during spermatogenesis- lose androgen receptor (AR) expression or androgen responsiveness; thus leaving them ill-suited for any further research regarding androgen regulation. The Immortalized Mouse Sertoli Cells (ST38c) eliminate this barrier with their continuous expression of endogenous AR. These cells are perfectly suited for investigating androgen receptor signaling in sertoli cell function in order to determine both molecular and cellular mechanisms.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Quantity	1x106 cells / 1.0 ml
Tissue	Reproductive
Morphology	Spindle shaped
Species	Mouse (M. musculus)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.ST38c cells must be grown in Prigrow III (TM003) base medium supplemented with a final concentration of fetal bovine serum (TM999)* to a final concentration of 5% and a final concentration of Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0
Quality Control	1. Androgen receptor mRNA was confirmed via RT-PCR while western blot confirmed AR protein expression. 2. Androgen receptor regulated genes were identified via microarray assays. 3. SV40 Tag expression confirmed through fluorescence microscopy

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SKU	Supplier No.	Your price	Quantity
BHC10900854	T0806	$3950		Add to Cart

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