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Immortalized Mouse Olfactory Bulb Cells (OBC13)

SKU : BHC10900784

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Applied Biological Materials (abm) Inc.

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Review(s)

Mfr.No.	T0237
Description	The olfactory bulb is located within the vertebrate forebrain, in which it functions to receive nerve endings/input from the nasal cavity. The sense of smell is associated with the nerve tissues of the olfactory bulb. Immortalized Mouse Olfactory Bulb Cells (OBC6) is immortalized with non-replicating retrovirus carrying the avian Myc gene. It is a sister derivative from the Immortalized Mouse Olfactory Bulb Cells (OBC1) (T0235). These cells are selected with the presence of G418. It is a valuable in vitro tool for studies relating to the functions and the biology of the olfactory bulb.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Brain
Morphology	Flattened\|Dendritic processes
Population Doubling	48 - 58 hours
Species	Mouse (M. musculus)
Propagation	This cell line needs to be cultured in Poly-L-Lysine coated flasks (TM061). The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, G418 (G271) to a final concentration of 0.3 mg/ml and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control	1) Resistance to 0.3 mg/ml G418, 2) Southern or Northern blot analysis of the viral integration site, 3) immunocytochemical analysis for markers

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SKU	Supplier No.	Your price	Quantity
BHC10900784	T0237	$3950		Add to Cart

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