Cell division cycle 25 (CDC25) family of phosphatases consists of CDC25A, CDC25B and CDC25C in which regulates the cell cycle progression, and thus are important for the activation of cell cycle checkpoints in response to DNA damage. CDC25A degradation has been associated with cell cycle arrest. Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) Cells were isolated from the Cdc25afl/fl/Cre mice in which the CDC25A gene is flanked with loxP inserted at exons 1-3 with the Krt14 promoter-driven Cre recombinase (Cre ) in which the expression of CDC25A is absent. abm also provides the Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) Cells (T0299).
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Quantity
1x106 cells / 1.0 ml
Tissue
Adipose
Morphology
Cobble-stone
Species
Mouse (M. musculus)
Propagation
Use of PriCoat T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Thaw the cells with PriGrow XI (TM011) + 8% FBS + 60 µM CaCl? (added fresh each time) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 8% CO? for optimal attachment. After 24h, change media to PriGrow XI (TM011) + 8% chelexed FBS + 1.4% FBS + 5 ng/ml rhFGF7 (Z100595) (freshly added) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 8% CO?Steps to thaw the cells:1. Thaw the cells in RT water until only a few ice crystals are left2. Put the cells in 5 ml of RT PriGrow XI (TM011) + 8% FBS3. Centrifuge the cells 1500 rpm for 5 min (need this step to remove inhibitors for attachment)4. Resuspend the pellet in RT Prigrow XI (TM011) + 8% FBS + 1% P/S with freshly added 60 µM CaCl? and put the cells in a culture vessel at 37.0°C, 8% CO?5. After 18h-24h, change to PriGrow XI (TM011) + 8% chelexed FBS + 1.4% FBS + 5 ng/ml rhFGF7 (Z100595) (freshly added) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 8% CO? for growth of the cells.
Quality Control
Immunoblot for the presence of CDC25A was conducted.