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Immortalized Mouse Intestinal Crypt Stem Cells (iMICs)

SKU : BHC10900755

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0564
Description	Intestinal epithelial cells provide a protective barrier against bacteria, antigens, and toxic substances which enters the body through the luminal tube. The cells are regulated to differentiate and proliferate to establish their protective function. Subepithelial intestinal myofibroblasts have been indicated to have a role in regulating intestinal epithelial cells. The Immortalized Mouse Intestinal Myofibroblasts are isolated from C57BL/6J mouse colonic muscosa and transfected with SV40 large T antigen. It retains primary myofibroblastic phenotypes, and expresses alpha-smooth muscle actin, vimentin, and type I collagen. Immune response-related proteins, such as TLR-4, CD14, MD2, and IkappaBalpha are also detected. Additionally, these cells are reactive to lipopolysaccharide (LPS) treatment, enabling an inflammatory response causing the degradation of IkappaBalpha and p38 MAPK phosphorylation. It is a valuable tool in research involving intestinal injury, fibrosis, inflammation, tissue repair, and immune regulation in intestinal tissue.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	alpha-smooth muscle actin, vimentin, type I collagen
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Colon
Morphology	Spindle shaped
Population Doubling	25 - 35 hours
Species	Mouse (M. musculus)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control	Western blot and immunofluorescence staining were used to determined cell markers and protein expression.

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SKU	Supplier No.	Your price	Quantity
BHC10900755	T0564	$2750		Add to Cart

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