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Immortalized Mouse Epidermal Keratinocytes (CDC25A F7)

SKU : BHC10900768

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Applied Biological Materials (abm) Inc.

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Description
Review(s)

Mfr.No.	T0299
Description	Cells were derived from hypothalamic region of E14.5 mouse brain and has been propagated for at least 25 passages (more than 100 duplications). The cells maintain high neurogenic potential and can generate neuronal cells expressing some hypothalamic peptides. Useful tool to investigate in vitro the mechanisms possibly involved in development, differentiation, and fate of hypothalamic neurons with neuroendocrine features.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period.
Expression Level	Arnt2 and Mash1 (Immunofluorescence); Nestin, Sox2, and Pax6 (RT-PCR)
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Brain
Population Doubling	24 hours
Species	Mouse (M. musculus)
Propagation	PriGrow X Series Medium (TM0861) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?. Thaw in T25, subculture with Accutase:PBS (ratio 1:1) for 3 minutes.

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SKU	Supplier No.	Your price	Quantity
BHC10900768	T0299	$3950		Add to Cart

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