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Immortalized Mouse Carotid Artery Endothelial Cells - hTERT

SKU : BHC10900747

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T9215
Description	The Immortalized Mouse CD4+ CD8+ T Cells (MOHITO) has characteristics of an immortalized cell line, but are not immortalized by any immortalization reagents. It expresses the Notch1 and Jak1 mutations, as well as TCR rearrangement, both of which are characteristics to human T-cell acute lymphoblastic leukemia (T-ALL). The cells are derived from CD4+ CD8+ T cells and are interleukin-7 (IL-7) dependent. The presence of IL-7 and IL-2 are required to activate the JAK/STAT signaling pathway. Transfection with BCR-ABL1 or mutant JAK1 transforms the cells to become IL-7 independent for proliferation. These cells are able to induce T-ALL-like disease when injected into healthy Balb/c mice. This cell line represents a novel model system to study T cell-specific protein signaling and inhibition mechanisms and oncogenic mutations, moreover they can be utilized in in vitro and in vivo pharmaceutical studies.Depending on the culture conditions and number of passages, the cells can become more CD8 single positive and lose CD4 expression. If CD4/CD8 double positive cells are needed, users will need to sort the cells regularly to select for the desired immunophenotype
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Growth Properties	Suspension
Quantity	1x106 cells / 1.0 ml
Tissue	Blood
Morphology	Round
Population Doubling	25 - 35 hours
Species	Mouse (M. musculus)
Propagation	Grow cells in 6-well plate (P0100) with the following conditions. The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 20%, mouse IL-2 to a final concentration of 5ng/ml, mouse IL-3 to a final concentration of 1ng/ml, mouse IL-7 to a final concentration of 10ng/ml and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.It's highly recommended to retain minimum 20% conditioned medium in each passage to ensure growth and propagation of cells.Do not use heat-inactivated FBS for cell culture unless specified otherwise.This cell line must be maintained at 400,000-800,000 cells/ml and should be split every 2-3 days. Cultures split lower than 300,000 cells/ml may result in slower cell propagation or a decrease in cell viability. When splitting the cells, avoid complete replacement of media. Instead retain approximately 1/5 of the conditioned media to maintain cell propagation speed and health.
Quality Control	1) Flow cytometry to analyze CD4+ CD8+ phenotype; 2) PCR assay for T cell receptor expression; 3) Sequence analysis to identify point mutations in Notch1 and Jak1

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SKU	Supplier No.	Your price	Quantity
BHC10900747	T9215	$3950		Add to Cart

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