Water and electrolyte homeostasis is largely regulated by the human proximal tubule; however this complex system has been hard to study particularly in relation to specific genes and their influence on renal physiology and pathophysiology. Immortalized Human Proximal Convoluted Tubule Cells (HPCT-05wt) is a potential model to acquire detailed physiological information about cell behaviour in response to hormones, growth factors, nutrients, and other external stimuli.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
NBCe1-A, NHE3
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Kidney
Population Doubling
24 - 48 hours
Species
Human (H. sapiens)
Propagation
Cells are grown on Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 mum (Millipore) that are lightlycoated with a 20% dispersion of rat-tail collagen (in 60% ethanol) plus 1 mug/mL laminin.The basemedium for this cell line is Prigrow IV medium available at?abm, Cat.No.?TM004. To make the complete growth medium,add the following components to the base medium: fetal bovine serum (TM999)* to a finalconcentration of 5%, HEPES (Sigma) to a final concentration of 15 mM, sodium selenite (Sigma) to a finalconcentration of 20 nM, ascorbic acid-2-phosphate (Sigma) to a final concentration of 50 mM, transferrin (Sigma)to a final concentration of 5 mug/mL, Insulin Solution (TM053) to a final concentration of 5 mug/mL, mouseEGF (Sigma) to a final concentration of 10 ng/mL, dexamethasone to a final concentration of 4 mug/mL, T3 (Sigma)to a final concentration of 1 nM, and Penicillin/Streptomycin Solution (G255) to a finalconcentration of 1%.Change media every 2-3 days by first removing it from the bottom of thefilter and then from the top. Add fresh medium first to the top and then to the bottom.Carbondioxide (CO2): 5%, Temperature: 37.0°C.* Do not useheat-inactivated FBS for cell culture unless specified otherwise.
Quality Control
1) Western blot
Reviews of Immortalized Human Proximal Tubule Cells (HPCT-03ts)