The Immortalized Human Gastric Cells (KMU-CS12) were derived from isolated gastric cell clones developed from gastric biopsy samples and were spontaneously immortalized in subsequent cultures. The growth of this cell line was found to be significantly faster than its parental gastric cells. This cell line shows cancer cell phenotypes with tumorigenic ability as tested in immunodeficient nude mice. Another unique characteristic of the Immortalized Human Gastric Cells (KMU-CS12) is the high expression of homeobox genes: HOXA4, HOXA5, HOXA7, HOXA9, and HOXA13. This makes the cell line useful in the construction of HOXA genes knockdown models as well as in research focusing on the chromosomal mutations in HOXA gene family. The Immortalized Human Gastric Cells (KMU-CS12) is also valuable in cancer research particularly in the etiology and studying new therapeutic strategy for gastric cancer.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
Oct-4, Musashi-1, TFF-1, CK8, CK18, EMA
Quantity
1x106 cells / 1.0 ml
Tissue
Stomach
Morphology
Polygonal
Population Doubling
60 - 70 hours
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Keratinocyte-SFM (Gibco-Invitrogen Corporation). To make the complete growth medium, add the following components to the base medium: N-acetyl-Lcysteine to a final concentration of 2 mmol/l, L-ascorbic acid 2-phosphate to a final concentration of 0.2 mmol/l and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C
Quality Control
1) Immunocytochemical staining to assess gene expression; 2) Cell proliferation and differentiation assay; 3) Spectral karyotype analysis (SKY)
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