The Immortalized Human Cranial Periosteal Cell Line (TAg58) is the first human cranial periosteal cell line isolated from a healthy adult patient biopsy and immortalized by lentiviral transduction with the large SV40 T antigen.The following are key characteristics that have been observed for the Immortalized Human Cranial Periosteal Cell Line (TAg58) (Alexander et al., 2015): Maintains long-term cell proliferation and osteogenic differentiation potentialShows stronger calcium phosphate mineralization than parental primary cellsMaintains similar expression patterns for most surface antigens as parental primary cellsExpresses higher levels of stem cell surface markers, CD146, and MSCA-1 (mesenchymal stem cell antigen-1), as well as higher constitutive mRNA levels of key osteogenesis factors (e.g. Osteocalcin, alkaline phosphatase, runx-2) compared to parental primary cellsThe Immortalized Human Cranial Periosteal Cell Line (TAg58) represents a suitable experimental platform for studying periosteal cell biology, bone formation, and bone regeneration. Depositor Profile: Prof. Dr. Dorothea Alexander-Friedrich, University Hospital TübingenIn Germany alone, more than 10,000 people are newly diagnosed with oral cancer every year resulting in tumor and in the most cases jaw bone resection. In order to reconstruct the resulting bone defects, an alternative to autologous bone transplantation is needed. The research laboratory of the Department of Oral and Maxillofacial Surgery of the University Hospital Tübingen focuses its research on the field of bone tissue engineering (TE). Read how their work with jaw and cranial periosteal cells translate into the generation of 3D TE-constructs and lead the way to biocompatible stem cell-based therapies.Read full profile.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
CD44, CD73, CD90, CD105, CD146, CD166, MSCA-1 and low expression of CD34; neomycin resistance (0.25 mg/mL G418)
Quantity
1x106 cells / 1.0 ml
Tissue
Bone
Morphology
Polygonal
Species
Human (H. sapiens)
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.The osteogenic differentiation medium for this cell line is complete culture medium plus the following supplements: 10 mM ?-glycerophosphate disodium salt hydrate (Sigma), 100 muM L-ascorbic acid 2-phosphate (Sigma), and 4 muM dexamethasone (Sigma).The osteogenic differentiation medium should be always prepared fresh.
Quality Control
1) Osteogenic differentiation experiments; 2) Western blot; 3) Flow cytometry; 4) Detection of cell mineralization by fluorescent OsteoImage staining; 5) Gene expression analysis
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