Owing to its multiple differentiation abilities, the Mesenchymal stromal cells (MSCs) holds the promise in tissue engineering and regenerative medicine, and displays important clinical potential for cell therapy. The Immortalized Human Cord Blood Mesenchymal Stromal Cells (cbMSC-hTERT) retains MSC specific surface markers (CD29, CD73, CD105, CD44 and HLA-ABC) as well as its differentiation capacities into cells of the mesodermal lineage, such as osteoblasts and adipocytes. In addition, karyotype analysis and CGH analysis show no chromosomal abnormality nor genomic deletion or amplifications, making this cell line suitable for stem cell research and translational study. Similarly, the Immortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP; Cat. T0017) exhibits comparable characteristics as cbMSC-hTERT with the addition of red fluorescent protein labelling. Transplantation of cbMSC-hTERT-RFP into the rat model with TBI demonstrates that these cells are readily attracted to the injury area and thus provides a valuable cell-based tool for stem cell transplantation and in vivo detection.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
CD29, CD73, CD105, CD44 and HLA-ABC
Growth Properties
Adherent
Quantity
1x106 cells / 1.0 ml
Tissue
Reproductive
Morphology
Fibroblast-like
Population Doubling
Population doubling time of 23.8 ± 1.9 h
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 20%, recombinant bFGF (Z101455) to a final concentration of 4 ng/ml and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control
1) RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells tumorigenic potential.
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