Immortalized Human Colonic Epithelial Cells (HCEC-1CT) is a human colonic epithelial cell (HCEC) line isolated from healthy adult patient biopsy and immortalized by transduction with the cyclin-dependent kinase 4 (Cdk4) and human telomerase reverse transcriptase (hTERT). HCEC-1CT cells express stem cell markers, such as LGR5, BMI1, CD29, and CD44, and retain multilineage epithelial differentiation capability. Thus, HCEC-1CT cells are capable of differentiating into various epithelial lineages of the colon and forming self-organizing cysts in 3D organotypic culture in Matrigel. During differentiation, HCEC-1CT cells express colon epithelial cell-specific markers such as A33 and mucin 1. Compared to most HCEC cell lines of malignant origin, HCEC-1CT is a valuable cytogenetically normal and nontumorigenic cell line for studying colon stem cell biology, differentiation as well as the initiation and progression of colonic diseases, such as colorectal cancer.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
LGR5, BMI1, CD29, and CD44In proliferation: vimentin, alpha-smooth muscle actinUpon growth arrest: cytokeratin 18, zonula occludens-1, mucins-1 and -2, antigen A33, and dipeptidyl peptidase 4
Quantity
1x106 cells / 1.0 ml
Tissue
Colon
Population Doubling
18 - 24 hours
Species
Human (H. sapiens)
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is a mix of Prigrow III medium (TM003) and Prigrow I medium (TM001), in 4:1 ratio respectively. To make the complete growth medium, add the following components to the base medium: cosmic calf serum to a final concentration of 2%, Recombinant Human EGF (Z100135) to a final concentration of 25 ng/mL, hydrocortisone (Sigma) to a final concentration of 1 mug/mL, Insulin Solution (TM053) to a final concentration of 10 mug/mL, Human Holo Transferrin (G599) to a final concentration of 2 mug/mL, sodium selenite (Sigma) to a final concentration of 5 nM, and Gentamicin (G272) to a final concentration of 50 mug/mL.Change media every 2-3 days.Carbon dioxide (CO2): 7%, Temperature: 37.0°C.* Do not use heat-inactivated serum for cell culture unless specified otherwise.
Quality Control
1) Western blot; 2) Immunocytochemistry; 3) Transmission electron microscopy; 4) Karyotype and DNA sequence analysis