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Immortalized Human Bronchiole Endothelial Cells

SKU : BHC10900481

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Applied Biological Materials (abm) Inc.

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Description
Review(s)

Mfr.No.	T0492
Description	Immortalized Human Brown Pre-Adipocytes are derived from infant brown adipose tissue. The vascular stromal cells were immortalized through microinjection of the vector pUC 18 carrying SV40 large and small T antigen under the control of a truncated human vimentin promoter. These immortalized cells express both PPAR and UPC1 and can be differentiated using a drug cocktail described below, resulting in terminally differentiaded brown adipocytes that display similar expression profiles and morphology to primary brown adipocytes. The functionality of this line may make it useful as an in vitro model of brown adipocytes and preadipocytes study.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	Positive for Proliferator activated receptor gamma (PPARgamma) and Uncoupling protein 1. Negative for human fatty acid binding protein (aP2), lipoprotein lipase (LPL), beta3-adrenergic receptor (beta3-AR) and hormone sensitive lipase (HSL)
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Adipose
Morphology	Epithelial
Population Doubling	50 - 60 hours
Species	Human (H. sapiens)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 8%, GlutaMAX (Gibco) to a final concentration of 1%, 15 mM HEPES, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.For differentiation of pre-adipocytes to adipocytes, the following media composition is recommended: A base medium of Prigrow IV media from abm (TM004) with fetal calf serum to a final concentration of 5%, GlutaMAX (Gibco) to a final concentration of 1%, HEPES to a final concentration of 15 mM, Biotin to a final concentration of 33µM, Pantothenate to a final concentration of 17µM, Triiodothyronine to a final concentration of 1nM, Dexamethoasone to a final concentration of 100nM, Pioglitazone to a final concentration of 1µM, Insulin (Z101065) to a final concentration of 500 nM, IBMX to a final concentration of 0.25mM (for the first 4 days of differentiation) and 1% Penicillin/Streptomycin (G255). Differentiation of cells takes approximately 14 days. During this time, the cells should be washed with PBS and the differentiation media change every 2 days. Adipocytes do not freeze well. Thus it is suggested that these cells initially be expanded and back up vials produced for recovery purposes.
Quality Control	1) Compared preadipocyte and adipocyte expression after terminal differentiation via semi-quantitative RT-PCR (Figure 1). 2) Visualized morphology of differentiated and undifferentiated cells via phase contrast microscopy (Figure 2). 3) Compared the expression profiles of primary and differentiated t0729 cells (Figure 3).

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BHC10900481	T0492	$2750		Add to Cart

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