The Bone Marrow Mesenchymal Cells are important for the growth and differentiation of primitive haematopoietic cells, as well as for maintenance of leukaemic cells. The immortalized cell was created by infecting primary mesenchymal cells with retrovirus containing hTERT and GFP genes separated by IRES under the control of MSCV LTR (pCIneo-hEST2-HA). The Immortalized Bone Marrow Mesenchymal cells can support growth of leukaemic cell lines 380, REH, OP-1 and 697, in addition to support the expansion of CD34+ cells. This cell line is useful not only in supporting the growth of haematopoietic cells, but can also be used in studies concerning bone marrow microenvironment and environmental cues that regulate haematopoiesis.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
CD44, CD73; these cells also express markers of osteoblasts (alkaline phosphatise) and chondrocytes (incorporation of toluidine blue dye)
Quantity
1x106 cells / 1.0 ml
Tissue
Bone Marrow
Morphology
Spindle-shaped
Population Doubling
35 - 45 hours
Species
Human (H. sapiens)
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and hydrocortisone to a final concentration of 10-6 M.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control
1) Immunofluorescence and RT-PCR were used to confirm the presence hTERT expression; 2) PCR based TRAP was used to assay telomerase activity; 3) Neo concentration not determined for selection use but cells were sorted for GFP+ populations
Reviews of Immortalized Human Bone Marrow Basophils