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Immortalized GFP-Expressing Mouse Primary Aortic Fibroblasts

SKU : BHC16403522

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Cell Biologics, Inc.

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Mfr.No.	C57-6075IM.GFP
Description	Immortalized GFP-Expressing Mouse Primary Aortic Fibroblasts from Cell Biologics are isolated from the aortic tissues of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. Immortalized GFP-Expressing Mouse Primary Aortic Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Type	Fibroblasts
Cell Models	Immortalized, GFP
Media	Complete Fibroblast Medium w/ Kit 500 ml
Tissue	Aortic
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse

Applications	Cardiovascular Research

Product Use	Immortalized GFP-Expressing Mouse Primary Aortic Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16403522	C57-6075IM.GFP	0.5x106 Cells	$3200		Add to Cart

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