The Immortalized Atherosclerotic Plaque Human Smooth Muscle Cell Line (pdSMC2A), is derived from explant cultures of surgical specimen obtained from a patient with diseased atherosclerotic palques in carotid artery. Cells were immortalized with pLXSN-HPV-16 E6/E7 retrovirus and TRAP assays confirmed telomerase activity. Norther analysis confirmed the presence of ?-smooth muscle-actin mRNA in pdSMC2A. Cells appear spindle-shaped with hill-and-valley organization patterns reminiscent of SMCs
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 20% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Source Organ
Blood Vessel
Shipping
Dry Ice
Storage
liquid nitrogen or -180C
Reviews of Immortalized Atherosclerotic Plaque Human Smooth Muscle Cell Line (pdSMC2A)