JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Cells / Cell Lines

IKCA1 (KCNN4) - HEK293 Cell Line

SKU : BHC18200232

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	90330
Description	Stable, recombinant HEK293 cell line expressing human IKCA1, also known as KCNN4 (Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4, GenBank Accession No. NM_002250. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Background	IKCA1 is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes.
Application	Drug compound screening for activators or inhibitors of IKCA1 Functional assays for IKCA1 Efficient antigen for mouse immunization
Biological Activity	N-terminal FLAG-tagged human IKCA1 has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. Functional analysis of IKCA1 channel in the stable recombinant HEK293 was accomplished by using the membrane potential-sensitive fluorescence dye DiBAC4(3). When IKCA1-expressed HEK293 cells were stimulated with calcium-specific ionophore ionomycin to raise the intracellular level of Ca²⁺, IKCA1 channel was activated, leading to hyperpolarization and a decrease in cell fluorescence in the functional fluorescence assay with DiBAC4(3). This decrease of cell fluorescence was blocked by the IKCA1 channel inhibitor, clotrimazole (CLT).
Host Cell	HEK293
Species	human
Synonyms	IKCA1, KCNN4, Intermediate conductance calcium-activated potassium channel protein 4, IK1, hKCa4, hSK4
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Ghanshani S et al. (2000). J Biol Chem. 275(47): 37137-37149. 2. Hoffman JF et al. (2003). PNAS 100 (12): 7366-7371. 3. de-Allie FA et al. (1996). Br J Pharmacol. 117(3):479-487. 4. Terstappen GC et al. (2003). Neurosci Lett. 346(1-2):85-88.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHC18200232	90330	2 vials	$9900		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.