Human Primary Tracheal Smooth Muscles Cells are isolated from the trachea from single donors and stain positive for smooth muscle ?-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, these cells are suitable for in vitro studies of asthma and other pulmonary diseases.
Disease
Normal
Density
7,500 - 10,000 cells per cm2
Expression Region
smooth muscle alpha actin
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Morphology
Multipolar
Organism
Homo sapiens
Population Doubling
10 PD.
Species
Human
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4013) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Source Organ
Trachea, Airway/Lung
Shipping
Dry Ice
Storage
-180°C
Reviews of Human Primary Tracheal Smooth Muscle Cells