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Human Primary Trabecular Meshwork Cells

SKU : BHC10901853

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T4182
Cell Type	Primary Cells
Biosafety Level	2
Disclaimer	1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Disease	Normal
Donor History	Normal tissue
Growth Properties	Adherent, epithelial
Growth Conditions	PriCoat?T25 Flasks (G299) coated with Poly-L-lysine?oating are required for optimal cell adhesion and growth. PriGrow X Series Medium (TM4182) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.
Quantity	5x10⁵ cells / 1.0 ml
Tissue	Eye
Morphology	Polygonal
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Species	Human
Propagation	Use of PriCoat^TM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4182) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Source Organ	juxtacanalicular and corneoscleral regions of human eye, Eye

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media and resuspend cells, dispense into a T25 culture flask. Do not centrifuge cells.?br />4. Incubate the cells at the recommended conditions.
Subculture Protocol	To detach cells, use a 1:1 solution of 0.05 % Trypsin-EDTA:PBS. Pre-warm all reagents to room temperature.Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.05 % Trypsin-EDTA:PBS (1:1)?o the culture vessel. 2. Observe the cells under a microscope to confirm that cells have detached and rounded. Collect the culture suspension in a 15ml falcon tube and incubate at 37? for 1 minute.?br>3. Neutralize 0.05 % Trypsin-EDTA:PBS (1:1) with an equal amount of Trypsin Neutraliziing Solution?nd centrifuge at 1000 rpm for 5 minutes.?br>4. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh Trypsin Neutralizing Solution?nd centrifuge at 1000 rpm for 5 minutes.?br>5. Re-suspend the cell pellet in pre-warmed recommended growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. 7. Cells can be passged up to 12 times.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10901853	T4182	5x10^5 cells / 1.0 ml	-		Add to Quote

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