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Human Primary Bladder Epithelial Cells

SKU : BHC16400028

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Cell Biologics, Inc.

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Mfr.No.	H-6215
Description	Human Primary Bladder Epithelial Cells fromCell Biologics are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin	Human
Cell Type	Epithelial Cells
Application	Human Primary Bladder Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Appropriate safety procedures should always be used with this material. Although Human Primary Alveolar Epithelial Cells are isolated from normal human tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen	0.5×10⁶cells
General Information	Human Primary Bladder Epithelial Cells from Cell Biologics are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 2-4 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Bladder

Product Use	Human Primary Bladder Epithelial Cells from Cell Biologics are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400028	H-6215	Frozen Vial	$610		Add to Cart

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