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Human CD8+ Cytotoxic Killer T Cells

SKU : BHC10900045

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T4121
Cell Type	Primary Cells
Biosafety Level	2
Disclaimer	1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Disease	Normal
Donor History	Normal tissue
Expression Region	CD8+
Gender	Donor Info Not Disclosed
Growth Properties	Suspension, round
Growth Conditions	PriGrow X Series Medium (TM4121) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.
Quantity	5x105 cells / 1.0 ml
Tissue	Blood
Morphology	Polygonal
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Species	Human (H. sapiens)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, L-glutamine to 2mM and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Applications Range	Research Use Only.
Applications	For Research Use Only

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 10ml of pre-warmed, complete growth media. Centrifuge cells at 300xg for 10 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and perform a cell count.?br />6. Refer to the "Subculturing Protocol" Section for information regarding T Cell Expansion.
Subculture Protocol	To expand T cells: 1. Seed 5.0x105?ells in a 24-well plate with 1ml of TM4121 supplemented with?00 IU/mL IL-2.?br>2. Activate T cells with antibodies capable of binding CD3 and CD28 as per manufacturer protocols.?br>3. Incubate the cells at the recommended conditions for up to 3 days.?br>4. On day 2 or 3, transfer cells into larger culture vessels, as per cell density. Centrifugation is not required; simply transfer the culfutre suspension and add additional media as required. Supplement with additional?L-2 to a final concentration of?00 IU/mL.?br>5. Perform a viabile cell count on day 5 and subculture cells as per steps 1 to 3, above. 6. Continue to expand T-cells by repeating the subculture procedure every 3 days. Do not wait longer than 3 days between subcutlures.?br>7. Cells are expected to expand for 9-12 days upon activation before entering a resting phase.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900045	T4121	5x10^5 cells / 1.0 ml	-		Add to Quote

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