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hERG (Kv11.1) - HEK293 Recombinant Cell line

SKU : BHC18200045

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BPS Bioscience

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Mfr.No.	60619
Description	Human ERG channel has been stably expressed in HEK293 cell line and its expressionwas confirmed by Western blotting. The channel activity of hERG was characterized by a fluorescence-based assay usingthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye. When hERG-HEK293 cells were pre-loaded withthallium-sensitive dye and stimulated with stimulus buffer containing potassium/thallium,thallium ions flowed through the open hERG channels into the cells and bound the dye,generating a fluorescent signal. The hERG channel activity in hERG-HEK293 cells wasblocked by hERG channel blockers, cisapride or dofetilide, causing the fluorescentsignal triggered by thallium influx to drop to the basal level. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM MycoplasmaDetection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO
Background	hERG (human ether-a-go-go-related gene) encodes the alpha subunit of a potassium ion channel, Kv11.1. It contains six transmembrane alpha-helices with a re-entrant "poreloop" between the fifth and the sixth transmembrane helices. This ion channel is best known for its contribution to the electrical activity of the heart that coordinates the heart's beating. When this channel's ability to conduct electrical current across the cell membrane is inhibited or compromised, either by application of drugs or by raremutations, it can result in a potentially fatal disorder called long QT syndrome. A numberof clinically successful drugs in the market exhibit the potential to inhibit hERG, andcreate a concomitant risk of sudden death as a side-effect, which has made hERGinhibition an important off-target that must be avoided during drug development.
Biological Activity	The channel has been validated for activity using a fluorescence-based assay that usesthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye.
Host Cell	HEK293
Species	Human
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc.

Product Format	aqueous solution containing DMSO
Storage Stability	Immediately upon receipt, store vials in liquid nitrogen.

Reference	1. Beacham, D.W. et al. (2010). J. Biomol. Screen. 15(4):441-446 2. Murphy, S.M. et al. (2006) J. Pharmacol. Toxicol. Methods. 54(1):42-55

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200045	60619	2 vials	$1996.50		Add to Cart

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